Frequency of donor TNF-alpha gene polymorphism in patients with graft versus host disease following hematopoietic stem cell transplantation.
- Author:
Jing-Bo WANG
1
;
Han-Yun REN
;
Dan LI
;
Qian SUN
;
Dao-Pei LU
Author Information
1. Institute of Hematology, The People' Hospital of Peking University, Beijing 100044, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Blood Donors;
Chromatography, High Pressure Liquid;
methods;
DNA;
chemistry;
genetics;
DNA Mutational Analysis;
Female;
Gene Frequency;
Genotype;
Graft vs Host Disease;
etiology;
Hematopoietic Stem Cell Transplantation;
adverse effects;
Humans;
Male;
Point Mutation;
Polymorphism, Genetic;
Tumor Necrosis Factor-alpha;
genetics
- From:
Journal of Experimental Hematology
2002;10(2):133-137
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of donor TNF-alpha gene polymorphism on severe acute and extensive chronic graft versus host disease (GVHD), TNF-alpha gene polymorphism was analyzed by denaturing high performance liquid chromatography (DHPLC) and DNA sequencing in twenty-one patients with III/IV degree acute GVHD and twenty-seven patients with extensive chronic GVHD. The results showed that the frequency of TNF-alpha-308 (G/A) significantly increased in patients with III/IV degree acute GVHD compared to 0/I degree aGVHD patients (8/21 vs 1/28) (P < 0.01) and the frequency of TNF-alpha-308 (G/A) is significantly higher in patients with extensive chronic GVHD than in patients without chronic GVHD (7/23 vs 1/17) (P < 0.05). However, the frequency of TNF-alpha-238 (G/A) does not significantly changed in patients with III/IV degree acute GVHD and extensive chronic GVHD. In conclusion, the TNF-alpha-308 (G/A) is likely to contribute to high risk for III/IV degree acute GVHD and extensive chronic GVHD.
