Expression and function of CD95/fas antigen and Bcl-2 on cord blood hematopoietic progenitor cells.
- Author:
Yan-Ping MA
1
;
Ping ZHOU
;
Juan XIAO
Author Information
1. Department of Hematology, Xiehe Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
Antibodies;
immunology;
Antigens, CD34;
analysis;
Apoptosis;
Female;
Fetal Blood;
metabolism;
Hematopoietic Stem Cells;
cytology;
metabolism;
Humans;
Proto-Oncogene Proteins c-bcl-2;
biosynthesis;
physiology;
fas Receptor;
biosynthesis;
immunology;
physiology
- From:
Journal of Experimental Hematology
2002;10(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
The cell-surface expression and functional status of the CD95/Fas antigen on primitive hematopoietic progenitors isolated from human cord blood were studied. The CD34(+) cells freshly isolated from cord blood displayed low CD95 expression, combinations of cytokines such as SCF+FL upregulated the expression of CD95 in CD34(+) cells. Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) further increased the CD95 expression induced by positive cytokines. The functional status of CD95-mediated apoptosis was analysed by incubation of CD34(+) cells in the presence of anti-CD95 monocloned antibody (McAb). The effect of anti-CD95 McAb was measured by viable cells counting, flow cytometry, LTC-IC and CFU-C assays. Viable cells and CFU-C numbers were 31.9 +/- 11.2 and 43 +/- 2.0 respectively, the rate of apoptosis was 42.9 +/- 12.4 in the presence of anti-CD95 McAb and TNF-alpha or IFN-gamma. However, growth factor deprivation or the early-acting cytokine such as SCF and FL cross-linking of CD95 lead to low apoptosis of CD34(+) cells. The correlation of increased intracytoplasmic levels of Bcl-2 and the presence of CD95 on surface of CD34(+) cells suggests that Bcl-2 may be involved in protecting against CD95-mediated apoptosis of cord blood CD34(+) cell.
