The effects of sera from patients with paroxysmal nocturnal hematoglobinuria on the growth of CD34(+) cells.
- Author:
Bing HAN
1
;
Yong-Ji WU
;
Zhi-Nan ZHANG
Author Information
1. Department of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Beijing 100730, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Blood Proteins;
pharmacology;
CD59 Antigens;
analysis;
Cell Division;
drug effects;
Cells, Cultured;
Hematopoietic Stem Cells;
drug effects;
pathology;
Hemoglobinuria, Paroxysmal;
immunology;
pathology;
Humans
- From:
Journal of Experimental Hematology
2002;10(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of sera on the growth of single CD34(+) cells from patients with paroxysmal nocturnal hematoglobinuria (PNH), sera from both PNH patients and normal individuals were added separately to the single cell culture system and semi-solid colony formation system. The growth of the normal CD34(+) and PNH CD34(+) CD59(+) and CD34(+) CD59(-) cells was evaluated. No growth difference was found for growth of the normal CD34(+) and PNH CD34(+) CD59(+) cells when PNH or normal sera were added to the culture media in either single cell culture or colony formation culture. While no difference was detected for single PNH CD34(+) CD59(-) cells growth when PNH or normal sera were added, more colonies were observed in semi-solid culture with PNH sera. A conclusion was reached that compared with those from normal controls, the sera from PNH patients had no significant influence on single hematopoietic stem/progenitor cells derived from normal subjects and from PNH patients, but the PNH sera might promote the colony formation of the CD34(+) CD59(+) cells in semi-solid culture
