Apoptosis of the allo-antigen specific T cells induced by CD34(+) cells transfected with exogenous gene FasL.
- Author:
Zhong-Wen LIU
1
;
Juan XIAO
;
Ping ZOU
Author Information
1. Institute of Hematology, The Affiliated Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD34;
analysis;
Apoptosis;
Cell Communication;
physiology;
DNA, Complementary;
genetics;
Fas Ligand Protein;
Humans;
Interferon-gamma;
pharmacology;
Membrane Glycoproteins;
genetics;
physiology;
Mice;
T-Lymphocytes;
cytology;
physiology;
Transfection;
fas Receptor;
biosynthesis
- From:
Journal of Experimental Hematology
2002;10(1):56-60
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the value of apoptosis of the allo-antigen specific T cells induced by Fas/FasL pathway in order to prevent GVHD in allo-transplant, the CD34(+) cells were transfected with FasL or not, used as effector cells, mixed with allo-antigen specific T lymphocytes with presence or absence of IFN-gamma or IL-2. After 5 days, apoptosis of T cells was detected by TdT nick end mediated dUTP labeling (TUNEL) and flow cytometry. The effects of IFN-gamma or IL-2 on apoptosis of CD34(+) cells of graft induced by Fas/FasL pathway observed as controls. The apoptosis incidence of T cells was (12.1 +/- 1.5)% when CD34(+) cells transfected with FasL were used as effector cells, that was much higher than that T cells with CD34(+) cells non-transfected (p < 0.01). In the presence of IFN-gamma or IL-2, apoptosis incidence reached to (20.1 +/- 2.3)% or (17.6 +/- 1.3)% respectively (p < 0.01). When sFasL was added to CD34(+) cells freshly isolated or induced with IFN-gamma or IL-2, the incidence or apoptosis of CD34(+) cells was (7.8 +/- 0.8)%, (18.7 +/- 1.6)% (p < 0.01) or (7.9 +/- 1.0)% (P > 0.05) respectively. The results suggest that it is possible to induce apoptosis of the allo-antigen specific T cells in grafts activated by allo-antigen by exogenous Fas ligand expressed on receptor cells and that may hopefully provide a new method to prevent GVHD
