Alteration of the Cell Cycle during the Differentiation of HL-60 Cells by Induction of Retinoic Acid
- Author:
Ying-Min LIANG
1
;
Shan-Shan JIANG
;
Rong-Li WU
;
Li LIU
;
Miao-Wang HAO
;
Zhong-Rong DENG
;
Zhi WANG
Author Information
1. Department of Hematology, Tangdu Hospital, The Fourth Military Medical University, Xian 710038, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2001;9(3):220-222
- CountryChina
- Language:Chinese
-
Abstract:
To study the alteration of the cell cycle during the differentiation of human myeloid leukemia cell line HL-60 induced with all trans-retinoic acid (RA), the flow cytometry was used to assay the various phases of cell cycle in HL-60 cells treated with RA. The results showed: (1) S + G(2)/M phase proportion kept relative invariability during the 48 hours incubation of HL-60 cells with RA, however, the proportion alteration of S-phase cells was associated with the RA concentrations. At 10(-6) mol/L RA, the proportion of S-phase cells appeared a temporarily increasing peak followed by persistent decrease of S-phase proportion. At 10(-5) mol/L RA, S-phase cell proportion only appeared the persistent decreasing tendency. (2) Re-culture of HL-60 cells without RA showed the decrease of S + G(2)/M and S-phase cells was associated with the increase of differentiated cells, but not all HL-60 cells were triggered into differentiation at the same time. Once the cells start to differentiate, even if there is no RA presence, the HL-60 cells were still differentiated until maturation. In conclusion, HL-60 cells are able to differentate and maturate after exposure to RA for a period of time. S-phase proportion is related to the concentrations of RA. Once the cells start to differentiate, even if there is no RA presence, the HL-60 cells still differentiatiated until maturation.
