Research of Typing for HLA-A, -B on Cord Blood Lymphocytes
- Author:
Jiong-Cai LAN
1
;
Qian SUN
;
Qian CHEN
;
Zhi-Mei ZHANG
;
Qiong CAO
;
Rong XIA
;
Da-Lin WU
;
Tao WU
Author Information
1. Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2001;9(3):251-255
- CountryChina
- Language:Chinese
-
Abstract:
Serological typing for HLA-A, -B has been used for a long time. Recently with the developing of molecular biology technologies, HLA-A, -B typing is now turning to genotyping methods. In our study, the capacity of PCR-SSP in solving problems in HLA-A, -B typing with serological methes was evaluated. With this aim the serological method was compared with PCR-SSP in 102 cord blood samples, and the results showed that 18.6% of 102 cord blood samples can't give a satisfactory detection, for 14 samples, give discrepant results with the 2 methods. It is mainly due to weak expression of HLA class I cord blood lymphocytes and the cross reaction of some antigens. About B 15 group, the further study was made, it was found that most of the B 15 splits is wrongly disassigned, especially among the B62-B75, B75/*1511(+)-B75/*1511(-), B46-*1511 antigens. It was concluded that DNA typing is more preferable than serological typing, about B 15 group, the subtyping or high resolution typing can be fulfilled at first in China.
