Liver transplantation with no-heatbeating donor supplied gaseous oxygen during cold preservation in pigs.
- Author:
Zhongxue FU
1
;
Stefan SAAD
;
Manfred NAGELSCHMIDT
;
Wenmiao HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cryopreservation; Heart Arrest; physiopathology; Hepatic Veins; Liver; physiopathology; Liver Function Tests; Liver Transplantation; Oxygen; therapeutic use; Reperfusion; Reperfusion Injury; prevention & control; Superoxide Dismutase; therapeutic use; Swine; Tissue Donors; Tissue Preservation; methods
- From: Chinese Journal of Hepatology 2002;10(1):17-19
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the availability of liver transplantation with non-heart beating (60 min) donors supplied gaseous oxygen by hepatic vein in cold preservation period in pigs.
METHODSThirty-six pigs were randomly divided into three recipient groups and three donor groups (group A-15 min of warm ischaemia, group B-60 min of warm ischaemia and group C-60 min of warm ischaemia with gaseous oxygen by hepatic vein during cold preservation period). OTL was performed after four hours cold preservation. Postoperative 5 days survival rate of the liver metabolic, function and inflammatory reaction were observed.
RESULTSAll animals in group A and C survived after reperfusion for 5 days, but animals in group B died within 3 hours postreperfusion. The damage of liver construction and function were found in group A and group B. There was no significant difference of the changes between group A and C.
CONCLUSIONSGaseous oxygen persufflation during cold preservation has important protective and resuscitative effect for liver from long-time no-heart beating donors. It was possible to become a promising method for liver transplantation with no-hearbeating donors.
