Effect of glycogen on calcium of donor liver during ischemia-reperfusion period.
- Author:
Yu WANG
1
;
Fuzhou TIAN
;
Lijun TANG
;
Darong HUANG
;
Xiaojun LI
;
Zhiliang YIN
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Triphosphate; metabolism; Animals; Calcium; metabolism; Calcium-Transporting ATPases; metabolism; Cytosol; chemistry; Female; Glycogen; metabolism; Liver Diseases; enzymology; metabolism; Liver Transplantation; physiology; Male; Models, Animal; Rabbits; Reperfusion Injury; enzymology; metabolism
- From: Chinese Journal of Hepatology 2002;10(1):21-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period.
METHODSDonor group (n=21) was divided into 3 subgroups randomly: Group A (n=7): fasting for 24 hours before harvesting; Group B (n=7): normal laboratory chew; Group C (n=7): normal laboratory chew plus glucose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content, ATP level, viability of Ca(2+)ATPase and plasmic free Ca(2+) concentration ([Ca(2+)]i) of liver tissue were measured.
RESULTSBefore cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca(2+)ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca(2+) concentration was lower in groups with higher glycogen content.
CONCLUSIONSDonor liver with high glycogen content can provide relatively sufficient ATP, maintain better Ca(2+)ATPase viability and prevent plasmic free Ca(2+) concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemia-reperfusion injury to the donor livers.
