Clone of a novel liver cancer associated gene and analysis of the secondary structures of the predicted protein.
- Author:
Zhengxu WANG
1
;
Guifang HU
;
Hongyang WANG
;
Mengchao WU
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Cell Line; Cloning, Molecular; Cytoplasm; chemistry; DNA, Neoplasm; Gene Expression Profiling; methods; Gene Expression Regulation, Neoplastic; Gene Library; Humans; Liver Neoplasms; genetics; Molecular Sequence Data; Neoplasm Proteins; biosynthesis; chemistry; genetics; Nuclear Proteins; Phosphorylation; Polymerase Chain Reaction; methods; Protein Structure, Secondary; Proteins; chemistry; genetics; Recombinant Proteins; biosynthesis; chemistry; genetics; Trans-Activators; Transcription Factors
- From: Chinese Journal of Hepatology 2002;10(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone a novel liver cancer associated gene, and to explore the molecular basis of liver cancer genesis.
METHODSUsing mRNA differential display polymerase chain reaction (DDPCR) and screening the human placenta cDNA library, we got a full-length cDNA of the gene. We prepared and purified the GST fusion protein and the special polyclonal antibody, engaged in the Western blot and immunohistochemical staining analysis, and analyzed the second structures and predicted the function of the protein by the computer soft.
RESULTSWe have got a full-length cDNA of the liver cancer associated gene and identified that the full-length cDNA of the gene could be expressed in 293 eukaryocytes by Western blot assay. We localized the target protein in cytoplasm using the immunohistochemical staining methods, and found two SH3 binding domains and several protein kinase phosphorylation sites by analyzing the second structures.
CONCLUSIONSWe have got a novel full-length cDNA of human liver cancer associated gene.
