Dynamic expression of tenascin in rat liver during liver fibrogenesis induced by CCl(4).
- Author:
Dingkang YAO
1
;
Shi LI
;
Xiantao KONG
;
Tingjun YE
;
Jiangao FAN
;
Lan ZHONG
;
Guoliang WANG
;
Liyan TIAN
;
Wensen WU
;
Mingsheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carbon Tetrachloride; Disease Models, Animal; Extracellular Matrix Proteins; biosynthesis; genetics; Image Processing, Computer-Assisted; Immunohistochemistry; In Situ Hybridization; Liver Cirrhosis; chemically induced; metabolism; Male; RNA, Messenger; biosynthesis; Rats; Rats, Sprague-Dawley; Tenascin; biosynthesis; genetics
- From: Chinese Journal of Hepatology 2002;10(1):40-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of tenascin in normal and fibrotic rat liver.
METHODSLiver fibrosis induced in rat with CCl(4) were divided into three stages: the stage of hepatic injury (4 weeks), early stage of hepatic fibrosis (8 weeks) and later stage of hepatic fibrosis (12 weeks). Tenascin expression in liver tissue was observed by immunohistochemical method and in situ hybridization using digoxigenin-labelled DNA probe.
RESULTSIn normal rat liver there was a weak staining for tenascin. In both liver injury stage and early stage of hepatic fibrosis, both mRNA signal and immunostaining for tenascin were significantly increased as compared to that in normal liver. In later stage of hepatic fibrosis, mRNA signal and immunostaining for tenascin were decreased compared with that in early stage of hepatic fibrosis. The cellular source of tenascin in liver mainly restricted in mesenchymal cells.
CONCLUSIONSTenascin is a component of the extracellular matrix of liver tissue. Plays a role in early matrix organization during liver fibrogenesis.
