Nucleic acid quantifying assay of hepatitis B virus.
- Author:
Ling ZHANG
1
;
Jianghua ZHU
;
Yun MA
;
Huarong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Alanine Transaminase; blood; Child; DNA, Viral; blood; Enzyme-Linked Immunosorbent Assay; methods; Female; Fluorescence; Hepatitis B Surface Antigens; blood; Hepatitis B e Antigens; blood; Hepatitis B virus; chemistry; genetics; Hepatitis B, Chronic; blood; Humans; Male; Middle Aged; Polymerase Chain Reaction; methods
- From: Chinese Journal of Hepatology 2002;10(1):49-51
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relation between the nucleic acid content of hepatitis B virus and hepatitis B virus markers, liver function and its pathological state.
METHODSThirty-nine patients suffering from chronic hepatitis B with positive HBsAg were performed liver puncture for histopathological examination. Fluorescence ration PCR analysis was used for HBV DNA quantifying assessment. Hepatitis B virus markers were detected by ELISA.
RESULTSSerum HBV DNA content was related to hepatitis B virus markers. There was no correlation between HBV DNA level and the Scheuer grade in patients with positive HBsAg, and no obvious correlation between serum glutamic pyruvic transaminase level and HBV DNA content.
CONCLUSIONSThere is a significant correlation between HBeAg and HBV DNA, but not between liver inflammation stage and serum HBV DNA content.
