Classification of genotyping hepatitis B virus with multiplex PCR.
- Author:
Jie YANG
1
;
Kangxian LUO
;
Yabing GUO
;
Lin DAI
;
Li YAN
;
Jinlin HOU
Author Information
- Publication Type:Journal Article
- MeSH: DNA Primers; Databases, Nucleic Acid; Genotype; Hepatitis B virus; classification; genetics; Humans; Polymerase Chain Reaction; methods; Sequence Alignment; methods
- From: Chinese Journal of Hepatology 2002;10(1):55-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple and practicable method to identify the different genotypes of hepatitis B virus (HBV).
METHODSBased on the alignment of 114 complete nucleotide sequence for HBV DNA of different genotypes, the specific sequence of each genotype was found. Six primer sets were designed for each of the six genotypes according to the genotype-specific sequence, and used separately for PCR. The genotype of HBV was identified according to the positive result of PCR. Three primer sets for B, C and D genotypes were added into a single tube for PCR reaction, and HBV was genotyped according to the length of the amplified DNA.
RESULTSThere was no difference in the genotyping result of PCR by single or multiplex primers, which was identical to the PCR-RFLP method.
CONCLUSIONSThis multiplex PCR method is simple, precise, sensitive, and easy to use.
