Morphology of Hypertrophic Scar Tissues and Expressions of Vascular Endothelial Growth Factor and Transforming Growth Factor Beta Activated Kinase 1 in These Tissues.
- Author:
Yu-fei ZHANG
1
;
Hou-zhong LI
;
Xue-yong WANG
;
Hong-chuang MA
1
;
Yan WU
1
;
Xiao-huan YUAN
1
;
Yan-hui CHU
1
Author Information
- Publication Type:Journal Article
- MeSH: Cell Shape; Cicatrix, Hypertrophic; Collagen; Fibroblasts; Humans; MAP Kinase Kinase Kinases; Transforming Growth Factor beta; Vascular Endothelial Growth Factor A
- From: Acta Academiae Medicinae Sinicae 2015;37(4):446-450
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the morphology of hypertrophic scar tissue and explore the expressions and distribution of vascular endothelial growth factor (VEGF) and transforming growth factor beta activated kinase 1(TAK1 )in these tissues.
METHODHematoxylin-eosin staining, Masson staining,immunofluorescence,and real-time polymerase chain reaction were used to detect the localization and expression of VEGF and TAK1 in 15 hypertrophic scar tissues and 10 normal skin tissues.
RESULTSMorphological observation showed that the dermal fibroblasts in hypertrophic scar were disorderly and densely arranged (compared to the normal skin). Immunofluorescence displayed that the expressions of VEGF and TAK1 in hypertrophic scar tissue were higher than in normal skin tissues. Real-time polymerase chain reaction showed the mRNA expressions of both VEGF and TAK1 were significantly higher in hypertrophic scar tissue than in normal tissue (P<0.01, P<0.05,respectively).
CONCLUSIONSHypertrophic scar tissue has higher collagen fibrosis degree and higher TAK1 and VEGF expressions than the normal skin. VEGF and TAK1 can be used as the reference indicators for the diagnosis and differential diagnosis of hypertrophic scar and serve as new therapeutic targets.
