Role of corticotropin releasing factor receptor 2 antisense oligodeoxynucleotide of hypothalamus in hypermetabolism in rats with severe burn.
- Author:
Zhong-yong CHEN
1
;
Shi-liang WANG
;
Xi PENG
;
Feng-jun WANG
;
Yun ZHAO
;
Pei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Burns; metabolism; Hypothalamus; metabolism; Male; Oligodeoxyribonucleotides, Antisense; metabolism; RNA, Messenger; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Receptors, Corticotropin-Releasing Hormone; genetics; metabolism
- From: Chinese Journal of Burns 2008;24(6):414-417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the role of corticotropin releasing factor receptor 2 antisense oligodeoxynucleotide (CRFR2ASO) of hypothalamus in hypermetabolism in rats with severe burn.
METHODSStainless-steel cannula were implanted into the 3rd ventricle. According to different medicine delivered into the 3rd ventricle, 30 SD rats with 30% TBSA full-thickness burn were divided randomly into burn control group (BC, with injection of 3 microL saline), CRFR1ODN group (with injection of CRFR1ODN 10 microg), CRFR1ASO group (with injection of CRFR1ASO 10 microg), CRFR2ODN group(with injection of CRFR2ODN 10 microg), CRFR2ASO group (with injection of CRFR2ASO 10 microg), with 6 rats in each group. Another 6 rats served as normal control (NC) and they received isotonic saline 3 microL instead. Different medicines were respectively delivered into respective group on 5, 6 post injury day (PID), then 3 microL gentian violet was introduced on 7 PID. Resting energy expenditure (REE) value and the expression level of hypothalamus CRFR2mRNA and CRFR2 protein were determined.
RESULTSREE value in BC, CRFR1ODN, CRFR1ASO, CRFR2ODN, CRFR2ASO groups was 11 840 +/- 987, 11 133 +/- 1100, 10 733 +/- 1338, 11 123 +/- 1321, 7563 +/- 890 kJx(m2)(-1)xd(-1), respectively, which were obviously higher than that in BC group [6641 +/- 526 kJx(m2)(-1)xd(-1), P < 0.05]. REE value in CRFR2ASO group was obviously lower than that in CRFR2ODN group (P < 0.01). The expression level of hypothalamus CRFR2 mRNA and its protein in BC group were increased after burn, which were obviously lower in CRFR2ASO group than NC group (P < 0.01).
CONCLUSIONCentral application of CRFR2ASO can downregulate the expression level of hypothalamus CRFR2 mRNA and its protein, and reduce hypermetabolism. Hypothalamus CRFR2 may mediate hypermetabolism in burn rats.
