Influence of human trefoil factor 1 (hTFF1) fusion protein on gastric mucosal lesion in mice after burn injury.
- Author:
Wei WU
1
;
Lin WANG
;
Yong SUN
;
Shang-jun LV
;
Yong ZHANG
;
Xi PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Burns; pathology; therapy; Disease Models, Animal; Gastric Mucosa; pathology; Humans; Mice; Recombinant Fusion Proteins; therapeutic use; Trefoil Factor-1; Tumor Suppressor Proteins; therapeutic use; Wound Healing
- From: Chinese Journal of Burns 2008;24(6):421-423
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of hTFF1 fusion protein on gastric mucosal lesion in mice after burn injury.
METHODSThe pET32alpha-hTFF1 vector were transfected into E.coli Origami B (DE3), and rhTFF1 fusion protein was expressed by IPTG induction. Mice were inflicted with 30% TBSA full-thickness burn, then were divided into burn treatment (BT, with treatment of rhTEF1 fusion protein by gavage), burn control (BC, with treatment of isotonic saline by gavage), and normal control (NC) groups according to block randomized design. Gastric mucosal lesion, including appearance of gastric mucosa, injury index, pathological change, were compared between BT and BC groups before and after burn injury, and above indices in NC group were also examined.
RESULTSThe rhTFF1 fusion protein was expressed with good specificity confirmed with Western blot analysis. The gastric erosion rate was 77.8% and 22.2% respectively in BC and T group. The injury index was 6.2 +/- 2.0 and 2.0 +/- 1.2 respectively in BC and T group. Above indices in C group were 0.
CONCLUSIONThe rhTFF1 fusion protein can be expressed in E.coli expression systems, which can obviously ameliorate gastric mucosal lesion in mice after burn injury.
