Relationship between drug resistance of Pseudomonas aeruginosa isolated from burn wounds and its mobile genetic elements.
- Author:
Xi-Hao HU
1
;
Xiao-Min XU
;
Zu-Huang MI
;
You-Fen FAN
;
Wei-Yun FENG
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; pharmacology; Burns; microbiology; DNA Transposable Elements; Drug Resistance, Multiple, Bacterial; genetics; Humans; Integrons; Microbial Sensitivity Tests; Plasmids; Pseudomonas aeruginosa; drug effects; genetics; isolation & purification
- From: Chinese Journal of Burns 2009;25(2):103-105
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the drug resistance of Pseudomonas aeruginosa (PA) isolated from burn patients wounds and its mobile genetic elements, including plasmid, transposon, and integron.
METHODSThirty-two strains of PA were isolated from wounds exudate of hospitalized burn patients in Ningbo No. 2 Hospital. PA drug sensitivity was determined using GNS-448 drug sensitivity card and K-B tests. The genetic markers of plasmid, transposon and integron including traA, traF, tnpA, tnpU, merA, int I 1 were amplified by PCR and verified by gene sequencing.
RESULTSDrug resistant rate of 32 PA strains to gentamicin, amikacin, cefoperazone/sulbactam, ciprofloxacin was 43.7%, 32.0%, 46.8%, 49.9%, respectively. PA drug resistant rates to piperacillin, cefotaxime, ceftazidime, cefepime, aztreonam, piperacillin/tazobactam, levofloxacin, imipenem and meropenem were all above 56.0%. Seventeen out of 32 PA strains were found to carry transposon and (or) integron genetic markers. One strain was positive for both tnpA and merA, 8 strains were positive for both merA and int I 1, 1 strain was only positive for tnpA, 2 strains were only positive for merA, and 5 strains were positive for int I 1 only.
CONCLUSIONSPA isolated from burn wounds of hospitalized patients in Ningbo No. 2 Hospital is seriously drug resistant, which may relate with its high positive rate of mobile genetic elements of transposon and (or) integron.