The interaction between secreted frizzled-related protein 2 and osteoblast-specific factor 2 in keloid.
- Author:
Zhen-Xiang WANG
1
;
Xi TAO
;
Shi-Rong LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Core Binding Factor Alpha 1 Subunit; genetics; metabolism; Genetic Vectors; Humans; Keloid; metabolism; Membrane Proteins; genetics; metabolism; Recombinant Fusion Proteins; metabolism; Transfection
- From: Chinese Journal of Burns 2009;25(2):126-128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo verify the interaction between secreted frizzled-related protein 2 (SFRP2) and osteoblast-specific factor 2 (OSF-2).
METHODSHA-tagged OSF-2 fusion protein recombinant vector pCMV-HA-OSF-2, which could express in mammal cells was constructed, then identified by enzyme-cutting and transfected into human kidney 293 (HK293) cells with or without Myc-SFRP2 recombinant eukaryotic expression vector pCMV-HA-SFRP2. The interaction between SFRP2 and OSF-2 was detected through coimmunoprecipitation and Western blotting.
RESULTSIn electrophoresis bath, target fragment of SFRP2 coding gene with 800 bp and target gene OSF-2 with 2500 bp could be seen respectively after enzyme-cutting, which showed that pCMV-Myc-SFRP2 and pCMV-HA-OSF-2 were constructed successfully. No HA-OSF-2 expression was detected after pCMV-Myc-SFRP2 or pCMV-HA-OSF-2 transfection. Whereas, HA-OSF-2 expressed by Myc antibody immunoprecipitation after pCMV-Myc-SFRP2 and pCMV-HA-OSF-2 co-transfection.
CONCLUSIONSHA-OSF-2 recombinant vector can express in mammal cells. Interaction exists between HA-OSF-2 and SFRP2.
