Effects of zinc on bone development in mouse embryo in vitro.
- Author:
Zengli YU
1
;
Yun LI
;
Ai ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Density; drug effects; Bone Development; drug effects; Calcium; metabolism; Embryo, Mammalian; drug effects; physiology; Extremities; embryology; Female; Insulin-Like Growth Factor II; Male; Mice; Proteins; metabolism; Zinc; pharmacology
- From: Chinese Journal of Preventive Medicine 2002;36(2):97-99
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThis study was designed to explore the effects of zinc on bone development.
METHODSForelimbs of mice with 16 d gestation were cultured by a rotating device.
RESULTSContents of OC and (45)Ca and activities of AKP in the bone tissues cultured at zinc-deficiency media and at media with 120 micro mol/L of Zn(2+) decreased significantly. Synthesis of OC, absorption of calcium and activities of AKP in the bone tissues cultures at media with 45 and 70 micro mol/L of Zn(2+) increased significantly. Radiograph of bone tissues showed that length of long bone cultured at zinc-deficiency media and at media with 120 micro mol/L of Zn(2+) shortened and their density reduced, and those cultured at media with 45 and 70 micro mol/L of zinc increased, as compared with self-control bone. Histological analysis showed the death of bone cells and loss of stroma in the bone tissues cultured at media with 120 micro mol/L of Zn(2+), and active proliferation and differentiation of bone cells, and secretion and synthesis of osteoid increased in the bone tissues cultured at media with 45 and 70 micro mol/L of zinc.
CONCLUSIONSAdequate supplementation of zinc could promote formation and development of bone tissues and deficiency or excess of zinc could alter their growth and development and normal metabolism.
