The microarray study on the stress gene transcription profile in human retina pigment epithelial cells exposed to microwave radiation.
- Author:
Xiuhong LIU
1
;
Hong SHEN
;
Yongliang SHI
;
Jingyuan CHEN
;
Yaoming CHEN
;
Ailing JI
Author Information
- Publication Type:Journal Article
- MeSH: Gene Expression Profiling; Gene Expression Regulation; radiation effects; Humans; Microwaves; Oligonucleotide Array Sequence Analysis; methods; Pigment Epithelium of Eye; cytology; metabolism; radiation effects; Retina; cytology; metabolism; radiation effects; Time Factors
- From: Chinese Journal of Preventive Medicine 2002;36(5):291-294
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the difference in stress and apoptosis related genes transcription between hTERT-RPE1 cells exposed to simulated microwave radiation and the cells with heat water bath, and the effects of microwave on gene transcription in cultured human retina pigment epithelial cells.
METHODScDNA microarray technique was used to detect the mRNA isolated from hTERT-RPE1 cells exposed to 2 450 MHz simulated microwave radiation and with heat water bath, respectively.
RESULTSAmong the 97 related aim genes, there were seven genes up-regulating its transcription, i.e., M31166 (2.52fold), L24123 (2.66fold), AF039704 (2.22fold), U67156 (2.07fold), AF040958 (2.13fold), NM-001423 (2.63fold) and NM-005346 (3.68fold). But, no notably down-regulating gene in transcription was detected.
CONCLUSIONSMicrowave could induce up-regulating in multiple stress and apoptosis related genes transcription in cultured human retina pigment epithelial cells, hTERT-RPE1 cells. Microwave radiation has unique effect itself in addition to its heat effect.
