Effects of selenium and zinc on rat renal apoptosis and change of cell cycle induced by fluoride.
- Author:
Rian YU
1
;
Tao XIA
;
Aiguo WANG
;
Xuemin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Cycle; drug effects; Cell Division; drug effects; DNA; drug effects; genetics; metabolism; Drug Antagonism; Flow Cytometry; In Situ Nick-End Labeling; Kidney; drug effects; pathology; Rats; Selenium; pharmacology; Sodium Fluoride; pharmacology; Zinc; pharmacology
- From: Chinese Journal of Preventive Medicine 2002;36(4):219-221
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study was conducted to study the effects of sodium fluoride (NaF) on rat renal apoptosis and proliferation, the antagonistic effect of selenium-zinc preparation (Se-Zn) to NaF.
METHODSWistar rats were provided with distilled water containing NaF (50 mg/L) and administered by gavage with different dosed of Se-Zn for six months. Kidney cell apoptosis and the cell cycle of proliferation were detected by TUNEL (TdT-mediated dUTP Nick End Labelling) and flow cytometry.
RESULTSNaF caused rat renal apoptosis, reduce the cell number of G(2)/M period in cell cycle and decrease the relative content of DNA significantly. Se-Zn inhibited the effects of NaF on apoptosis and increased the cell number of G(2)/M period in cell cycle, but failed to increase relative content of DNA.
CONCLUSIONIt was suggested that NaF could induce apoptosis and change the cell cycle in rat renal cells and Se-Zn could antagonize apoptosis and the changes of cell cycle induced by NaF.
