The expression of bcl-2 and bax genes during microcystin induced liver tumorigenesis.
- Author:
Zhijian HU
1
;
Hua CHEN
;
Yiwei LI
;
Lingyun GAO
;
Changsheng SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinogens; toxicity; Immunohistochemistry; Liver; drug effects; metabolism; pathology; Liver Neoplasms; chemically induced; metabolism; pathology; Mice; Microcystins; Peptides, Cyclic; toxicity; Proto-Oncogene Proteins; biosynthesis; Proto-Oncogene Proteins c-bcl-2; biosynthesis; Rats; Rats, Wistar; bcl-2-Associated X Protein
- From: Chinese Journal of Preventive Medicine 2002;36(4):239-242
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the molecular mechanism of microcystin (MC) induced liver tumorigenesis in rats.
METHODSThe two-stage-medium-term tumorigenesis theory was applied to establish the animal model, and the effect of MC in liver tumor formation was evaluated by the Albert gamma-GT methods, and then, the immunohistochemical technique and image analysis were used to study the expression of the bcl-2 and bax genes during tumorigenesis.
RESULTS(1) MC enhanced the formation of gamma-GT foci in liver (100%), which was significantly higher than the diethylnitrosamine (DEN) control group (22.22%) (P < 0.05). (2) MC decreased the expression of bax gene. The intensity and area of bax gene expression in the pure MC toxin group were 0.028 3 AODV and 0.007 3 ( micro m(2)/ micro m(2)) and in the DEN control group were 0.065 5 AODV and 0.024 4 ( micro m(2)/ micro m(2)), respectively. The intensity and areas of bax gene expression in the pure MC toxin group were significantly lower than those in the DEN control group (P < 0.05). (3) MC increased the expression of bcl-2 gene. The intensity and area of bcl-2 gene expression in the pure MC toxin group wee 0.097 7 AODV and 0.031 5 ( micro m(2)/ micro m(2)), respectively, and in the DEN control group were 0.046 0 AODV and 0.020 5 ( micro m(2)/ micro m(2)) respectively (P < 0.05).
CONCLUSION(1) MC can strongly promote liver tumorigenesis. (2) The changes of bcl-2 and bax gene expression possibly play an important role in the MC induced liver tumor formation.
