Application of reverse transcriptase polymerase chain reaction for surveillance of influenza virus in Beijing.
- Author:
Weixian SHI
1
;
Hailin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Orthomyxoviridae; classification; isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity
- From: Chinese Journal of Preventive Medicine 2002;36(6):382-385
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the RT-PCR method to type the influenza virus.
METHODSAfter amplifying the virus by cell culture, we carried out RT-PCR by using two pairs typing primers and four pairs subtyping primers to detect the influenza virus.
RESULTSIn those 23 samples which had cytopathologic changes, there were 10 positive strains detected by RT-PCR assay including seven A type (six H3N2 subtype and one H1N1 subtype) and three B type.
CONCLUSIONThis method is rapid, specific and sensitive and possesses great value for practical application in the surveillance of influenza virus.
