Effects of microcystins on cell cycle and expressions of c-fos and c-jun.
- Author:
Jin-ming ZHAO
1
;
Hui-gang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinogens; toxicity; Cell Cycle; drug effects; Cell Division; drug effects; Cells, Cultured; Cricetinae; Gene Expression; drug effects; Microcystins; Peptides, Cyclic; toxicity; Proto-Oncogene Proteins c-fos; biosynthesis; Proto-Oncogene Proteins c-jun; biosynthesis
- From: Chinese Journal of Preventive Medicine 2003;37(1):23-25
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the effects of microcystins on cell cycle and expressions of c-fos and c-jun, and explore the potential carcinogenic mechanisms of Microcystins.
METHODSMicrocystic cyanobacteria extraction (MCE) purified by Sep-Pak C(18) cartridge was added into the media and co-incubated with SHE cell for various periods. Immunohistochemistry assay was applied to detect the expressions of c-fos and c-jun at 1, 3, 6 hr time point, and cell cycle at 6, 12, 24 hr point were analyzed by flow cytometry respectively.
RESULTSSustained up-regulated expression of c-fos and c-jun were induced by MCE during the experimental period, and 5 - 6 folds increased expression were observed at 6 hour point after treatment. As much as 44.8 per cent of cells were induced to entry S-phase from resting G(0)/G(1).
CONCLUSIONUp-regulating the expression of transcript factor such as c-fos and c-jun thus to induce cell abnormal proliferation may be the potential carcinogenic mechanisms of microcystins.
