The effects of conjugated linoleic acid on the expression of invasiveness and metastasis-associated gene of human gastric carcinoma cell line.
- Author:
Yan-mei YANG
1
;
Bing-qing CHEN
;
Yu-mei ZHENG
;
Xuan-lin WANG
;
Jia-ren LIU
;
Ying-ben XUE
;
Rui-hai LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; pathology; Gene Expression; drug effects; Humans; Linoleic Acid; pharmacology; therapeutic use; Monomeric GTP-Binding Proteins; biosynthesis; genetics; NM23 Nucleoside Diphosphate Kinases; Neoplasm Invasiveness; prevention & control; Nucleoside-Diphosphate Kinase; RNA, Messenger; biosynthesis; drug effects; Stomach Neoplasms; pathology; Tissue Inhibitor of Metalloproteinase-1; biosynthesis; genetics; Tissue Inhibitor of Metalloproteinase-2; biosynthesis; genetics; Transcription Factors; biosynthesis; genetics; Tumor Cells, Cultured
- From: Chinese Journal of Preventive Medicine 2003;37(1):26-28
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on invasive ability of human gastric carcinoma cell line (SGC-7901) and to explore its possible mechanism.
METHODSReconstituted basement membrane invasion assay was used to evaluate invasive ability of cancer cells. Expression of TIMP-1, TIMP-2 and nm23-H(1) mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) in SGC-7901 cells.
RESULTSAt the concentrations of 200 micromol/L, 100 micromol/L and 50 micromol/L, c9,t11-CLA suppressed their reconstituted basement membrane invasion of SGC-7901 by 53.7%, 40.9% and 29.3%, respectively. c9,t11-CLA could induce the expression of TIMP-1, TIMP-2 and nm23-H(1) mRNA in SGC-7901 cells.
CONCLUSIONSThe invasion of SGC-7901 cells could be inhibited by c9,t11-CLA through reconstituted basement membrane. Anti-invasion action of c9,t11-CLA might be associated with induction of expression of TIMP-1, TIMP-2 and nm23-H(1) mRNA in tumor cells.
