The expression and activity of caspase-8 in the process of vitamin E succinate-induced apoptosis in human gastric carcinoma SGC-7901 cells.

Author: Yao LI ¹ ; Kun WU ; Wei-ping YU
Author Information

1. Nutrition and Food Department, Public Health College of Harbin Medical University, Harbin 150001, China.
Publication Type:Journal Article
MeSH: Apoptosis; drug effects; Caspase 8; Caspases; metabolism; physiology; Dose-Response Relationship, Drug; Humans; Stomach Neoplasms; enzymology; pathology; Tocopherols; Tumor Cells, Cultured; Vitamin E; analogs & derivatives; pharmacology
From: Chinese Journal of Preventive Medicine 2003;37(2):112-114
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the role of caspase-8 in vitamin E succinate (VES)-induced apoptosis in human gastric carcinoma cells.
METHODSThe expression and activity of caspase-8 were measured by the Western Blot method with fluorescence and DAPI staining.
RESULTSVES treatment induced the expression and activity of caspase-8 in a dose-dependent manner; the apoptosis ratio induced by VES was significantly decreased in SGC-7901 cells treated with caspase-8 inhibitor.
CONCLUSIONCaspase-8 plays an important role in SGC-7901 cell apoptosis induced by VES.