The effects of three plastic additives on the proliferation of MCF-7 cell.
- Author:
Zeng-li YU
1
;
Li-shi ZHANG
;
Pei-yu XU
;
De-sheng WU
Author Information
- Publication Type:Journal Article
- MeSH: Benzhydryl Compounds; Breast Neoplasms; pathology; Cell Division; drug effects; Cell Line, Tumor; Dibutyl Phthalate; toxicity; Environmental Pollutants; toxicity; Estrogens, Non-Steroidal; toxicity; Female; Humans; Phenols; toxicity
- From: Chinese Journal of Preventive Medicine 2003;37(3):150-153
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of environmental estrogens on the proliferation of breast cancer cell line MCF-7.
METHODSThe tested compounds were n-4-nonyphenol, Bisphenol A and dibutylphthalate. Human estradiol-dependent MCF-7 breast cancer cells were grown in DMEM medium containing 10% bovine serum. Five days before the addition of the test compounds, the cells were washed by phosphate-buffered saline, and the medium was substituted with a phenol red-free DMEM medium containing 5% dextral charcoal-stripped FBS. The respective test compound was added in fresh medium and the control cell received only the vehicle (ethanol). The proliferation of MCF-7 was analyzed by the MTT assay, (3)H-TdR incorporation assay and flow cytometry.
RESULTSCompared with the ethanol control, the proliferation and DNA synthesis of the test cells treated with n-4-nonyphenol (8 x 10(-7) mol/L, 96 h), Bisphenol A (8 x 10(-7) mol/L, 96 h) or dibutylphthalate (32 x 10(-6) mol/L, 96 h) treatment was markedly enhanced in a time-dependent and dose-dependent manner.
CONCLUSIONn-4-Nonyphenol, Bisphenol A and dibutylphthalate enhanced the proliferation of human breast cancer cell in vitro, which may demonstrate an estrogenic activity.
