Protecting action of acupuncture serum on calcium over-loaded neurons of the hippocampus in rats of ischemia-reperfusion.
- Author:
Jin-Ling ZHANG
1
;
Ying GUO
;
Rui-Wu LI
;
Cui-Hong LI
;
Ming-Fu LUO
Author Information
- Publication Type:Journal Article
- MeSH: Acupuncture Therapy; Animals; Blood Proteins; pharmacology; Brain Ischemia; metabolism; therapy; Calcium; metabolism; Cells, Cultured; Hippocampus; blood supply; drug effects; metabolism; Humans; Neurons; drug effects; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury; metabolism; prevention & control; therapy; Serum; chemistry
- From: Chinese Acupuncture & Moxibustion 2009;29(1):45-47
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of acupuncture serum on Ca2+ content in the cultured nervous cells of hippocampus after ischemia-reperfusion, so as to probe into humoral factors in acupuncture treatment.
METHODSThe neurons of the hippocampus from the new born rats were cultured for 9-11 days. Fluorescein-molecular probe Fluo-3 AM was used for staining of intracellular Ca2+. Fluorescent levels in the nervous cells cultured with the serum of the normal rats or the rats given electroacupuncture at "Baihui" (GV 20), "Zusanli" (ST 36), "Quchi" (LI 11) and "Sanyinjiao" (SP 6) for 2 weeks were determined by using a laser confocal microscope.
RESULTSAfter the normal serum was added, the intracellular Ca2+ fluorescent levels increased to 697 +/- 113 from 461 +/- 96, while after acupuncture serum was added, the Ca2+ fluorescent levels decreased to 584 +/- 103 from 673 +/- 108, indicating that after addition of acupuncture serum, the increased intracellular Ca2+ content could be decreased.
CONCLUSIONThere are some active substances in acupuncture serum which can obviously decrease intracellular Ca2+ content after ischemia-reperfusion, so as to provide a direct evidence for role of humoral factor in acupuncture treatment.
