The molecular mechanism between baicalin metal complexes and bovin serum album.
- Author:
Ming GUO
;
Xian TAN
;
Ying WANG
;
Xiao-yan GAO
;
Zhou-ling WU
;
Li-jun ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Coordination Complexes;
chemistry;
Electrodes;
Flavonoids;
chemistry;
Kinetics;
Nanotubes, Carbon;
Serum Albumin, Bovine;
chemistry;
Thermodynamics
- From:
Acta Pharmaceutica Sinica
2015;50(5):613-620
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, the new carbon nanotube modified glassy carbon electrode (F-CNTs/GCE) was prepared to establish a new method for studying the molecular interaction mechanism between baicalin metal complexes (BMC) and bovine serum album (BSA), and the principle of this method was discussed deeply. Under the physiological condition, the thermodynamics and kinetics properties of interaction between BMC and BSA were studied by cyclic voltammetry (CV) to inference their molecular effective mechanism. The results show that the presence of F-CNTs can accelerate the electron transfer, and better response signal was showed in the BMC/BMC-BSA system. The detection of interaction of BMC-BSA used new method show that BMC-BSA generates stable thermodynamically non-covalent compounds, and the obtained average binding sites of BMC-BSA were 1.7; the number of electron transfer in BMC/BMC-BSA reaction process was 2, and non electroactive supramolecular compounds of BMC-BSA were generated by this interacting reaction. The relevant research work provides a new way to study the molecular mechanism for the interaction of drugs with protein, and with a certain reference value for discussion on the non covalent interactions.
