Neuroprotective effect screening and the mechanism of 10 kinds of coumarin derivatives.
- Author:
Xiu-yun SONG
;
Jin-feng HU
;
Ming-na SUN
;
Gang LIU
;
Nai-hong CHEN
- Publication Type:Journal Article
- MeSH:
Animals;
Coumarins;
chemistry;
Free Radical Scavengers;
chemistry;
Inflammation;
Microglia;
drug effects;
Neurons;
drug effects;
Neuroprotective Agents;
chemistry;
PC12 Cells;
Rats
- From:
Acta Pharmaceutica Sinica
2015;50(6):697-701
- CountryChina
- Language:Chinese
-
Abstract:
The study reports the detection of neuroprotective effect of 10 kinds of coumarin derivatives and explores their possible mechanism. MTT method was used to screen the neuroprotective effect of 10 coumarin derivatives on neurotoxic agents (Aβ25-35 and rotenone) or OGD (oxygen-glucose deprivation). A compound with better protective effect was obtained. Then the effect of this compound on neurotoxic agents on PC12 was detected by the morphological observation. Furthermore, the effect of compound 3 on microglia with lipopolysaccharide (LPS) induced inflammation was detected. And the inflammatory factor was tested. Finally, direct free radical scavenging ability was detected. Compound 3 was found to be the best compound through three neurons toxic models. Not only compound 3 ameliorated cell viability reduced by three neurons toxic models, but also significantly inhibited the production of inflammatory factor (TNF-α and IL-1β). And its free radical scavenging ability is very good, especially the effect on superoxide anion, which is comparable with vitamin C. The significant scavenging effect of compound 3 on superoxide anion might be the mechanism of the neuroprotection. Compound 3 as a potential neural cell protective agent merits further investigation.
