Effect of Recombinant Human Epidermal Growth Factor Impregnated Chitosan Film on Hemostasis and Healing of Blood Vessels.
10.5999/aps.2014.41.5.466
- Author:
Sangshin LEE
1
;
Inwook JUNG
;
Seongcheol YU
;
Joon Pio HONG
Author Information
1. Department of Plastic and Reconstructive Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. joonphong@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Epidermal growth factor;
Chitosan;
Hemostasis
- MeSH:
Blood Vessels*;
Chitosan*;
Debridement;
Epidermal Growth Factor*;
Gelatin;
Hemorrhage;
Hemostasis*;
Humans;
Myocytes, Smooth Muscle;
Porifera;
Punctures;
Rats, Sprague-Dawley;
Ulcer;
Wounds and Injuries
- From:Archives of Plastic Surgery
2014;41(5):466-471
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Bleeding can be a problem in wound debridement. In search for an effective hemostatic agent, we experimented with a chitosan film combined with the recombinant human epidermal growth factor (rh-EGF), hypothesizing that it would achieve effective hemostasis and simultaneously enhance arterial healing. METHODS: Forty-eight Sprague-Dawley rats were used, and 96 puncture wounds were made. The wounds were divided into the following four groups: treated with sterile gauze, treated with gelatin sponge, treated with chitosan, and treated with chitosan combined with rh-EGF. Immediate hemostasis was evaluated, and arterial healing was observed histologically. RESULTS: Groups B, C, and D showed a significant rate of immediate hemostasis as compared to group A (P<0.05), but there were no significant differences among groups B, C, and D. Histologically, only group D showed good continuity of the vessel wall after 1 week. It was the only group to show smooth muscle cell nuclei of the vessel wall. CONCLUSIONS: We observed that chitosan has an effective hemostatic potential and the mix of rh-EGF and chitosan does not interfere with chitosan's hemostatic capabilities. We also identified enhanced healing of vessel walls when rh-EGF was added to chitosan. Further research based on these positive findings is needed to evaluate the potential use of this combination on difficult wounds like chronic diabetic ulcerations.
