The action mechanisms of Morus alba leaves extract for the treatment of diabetes based on plasma metabolomics.
- Author:
Tao JI
;
Li-li ZHANG
;
Xiao-chen HUANG
;
Shu-lan SU
;
Zhen OUYANG
;
Zhen-hua ZHU
;
Sheng GUO
;
Er-xin SHANG
;
Da-wei QIAN
;
Jin-ao DUAN
- Publication Type:Journal Article
- MeSH:
Alkaloids;
chemistry;
Amino Acids;
metabolism;
Animals;
Biomarkers;
blood;
Chromatography, High Pressure Liquid;
Diabetes Mellitus, Experimental;
drug therapy;
Flavones;
chemistry;
Flavonoids;
chemistry;
Metabolic Networks and Pathways;
Metabolomics;
Mice;
Morus;
chemistry;
Plant Leaves;
chemistry;
Sphingolipids;
metabolism;
Tandem Mass Spectrometry
- From:
Acta Pharmaceutica Sinica
2015;50(7):830-835
- CountryChina
- Language:Chinese
-
Abstract:
In order to evaluate the effect and mechanism of the mulberry leaf alkaloid, flavones, and polysaccharide intervention on diabetes, the overall metabolite profiling characteristics for the plasma of diabetic mouse was performed by using an ultra-performance liquid chromatography/electrospray-tandem mass spectrometry (UPLC-ESI-MS). The 8 potential biomarkers were found in diabetic mice plasma based on the data of MS/MS characteristics obtained from the UPLC-OrbitrapMS analysis, which mainly involved in sphingolipids, amino acid metabolic pathway. The principal component analysis showed that the normal group and model group were obviously distinguished and implied that metabolic disturbance was happened in diabetic mice plasma. The extracts of mulberry leaf flavonoids, polysaccharide, alkaloid had exhibited the effects of callback function for diabetic mice through regulating the amino acid metabolism and sphingolipid metabolism.
