UPLC-Q-TOF/MS analysis of phospholipids metabolite profiling in plasma of type 2 diabetes mellitus rat.
- Author:
Ping-yan ZENG
;
Yu ZHANG
;
Wen RUI
;
Xia WU
;
Yi-fan FENG
- Publication Type:Journal Article
- MeSH:
Animals;
Biomarkers;
blood;
Chromatography, High Pressure Liquid;
Diabetes Mellitus, Experimental;
blood;
Diabetes Mellitus, Type 2;
blood;
Metabolome;
Phospholipids;
blood;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry
- From:
Acta Pharmaceutica Sinica
2015;50(7):882-886
- CountryChina
- Language:Chinese
-
Abstract:
This study reported the analysis of plasma phospholipid metabolism of the rats and the pathological biomarkers between the type 2 diabetes model control group (MC) and the normal control group (NC). SD rats were randomly divided into 2 groups: NC and MC. To investigate state of plasma metabolite profiling in normal body, type 2 diabetes mellitus (T2DM) model group using UPLC-Q-TOF/MS which was used as analysis tool in this research. The compounds were identified by UPLC-Q-TOF/MS based on MS/MS fragment ions information, element composition in MassLynx 4.1 and the Lipid Maps database. The sign of two groups of samples in specific markers for screening was through a software package in R software (BioMark software). The results show that the pathological markers were mainly phosphatidylcholine (PC) and triglycerides (TG); the 2-acyl PC in the MC group was less more obviously than that in the NC group; high carbon number and high degree of unsaturation of the TG was reduced under the condition of type 2 diabetes. In the state of type 2 diabetes, metabolic changes occurred in rat plasma phospholipids obviously, which had a close relationship with the occurrence and development of T2DM.
