Mechanism of genuineness of Glycyrrhiza uralensis based on SNP of β-Amyrin synthase gene.
- Author:
Yi-mei ZANG
;
Yan-peng LI
;
Jing QIAO
;
Hong-hao CHEN
;
Chun-sheng LIU
- Publication Type:Journal Article
- MeSH:
Exons;
Genotype;
Glycyrrhiza uralensis;
classification;
enzymology;
genetics;
Intramolecular Transferases;
genetics;
Plant Proteins;
genetics;
Polymorphism, Single Nucleotide;
Polymorphism, Single-Stranded Conformational
- From:
Acta Pharmaceutica Sinica
2015;50(7):906-909
- CountryChina
- Language:Chinese
-
Abstract:
β-Amyrin synthase (β-AS) genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between β-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of β-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions (P < 0.001). The results of the experiment proved that different β-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.
