The expression of BmK AngM1 in Mut(s) and Mut(+) recombinants of Pichia pastoris.
- Author:
Qing-hua WANG
;
Lan LIANG
;
Jing-jing CHEN
;
Ting GONG
;
Qi HOU
;
Jin-ling YANG
;
Ping ZHU
- Publication Type:Journal Article
- MeSH:
Animals;
Arthropod Proteins;
biosynthesis;
Gene Dosage;
Pichia;
metabolism;
Recombinant Proteins;
biosynthesis;
Scorpion Venoms;
chemistry
- From:
Acta Pharmaceutica Sinica
2015;50(7):910-915
- CountryChina
- Language:Chinese
-
Abstract:
BmK AngM1 is a long-chain scorpion toxin purified from the venom of Buthus martensii Karsch. It has been reported to exhibit evident analgesic effect and low toxicity, and has the potential to be a novel analgesic drug. The BmKAngM1 gene was transformed into Pichiapastoris GS115. Mut+ and Mut(s) recombinant strains were screened by phenotype and Mut+ recombinant strains were used to detect BmK AngMl gene copy number in the real-time PCR. Expression of BmK AngM1 in the Mut+ recombinant strain was compared with that of the Mut(s) recombinant strain with the same single copy of BmK AngM1 gene under the same condition. The results indicated that the transcription level of BmK AngM1 gene in the Mut(s) recombinant strain was 2.7 fold of that in the Mut recombinant strain in the real-time PCR, and the expression of BmK AngM 1 in the Mut(s) recombinant strain was 1.5 fold of that in the Mut+ recombinant strain. Therefore, Mut(s) recombinant strain showed better ability to express BmK AngM1 than Mut+ recombinant strain.
