Effects of SUMO specific protease 1 on hPXR-mediated P-gp gene expression.
- Author:
Ying-mei LI
;
Chen-shu XU
;
Zhang-yan LI
;
Xiao CHEN
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter, Sub-Family B;
metabolism;
Cysteine Endopeptidases;
Endopeptidases;
metabolism;
Gene Expression;
Hep G2 Cells;
Humans;
Peroxisome-Targeting Signal 1 Receptor;
RNA, Messenger;
Receptors, Cytoplasmic and Nuclear;
metabolism;
Transcriptional Activation
- From:
Acta Pharmaceutica Sinica
2015;50(9):1192-1196
- CountryChina
- Language:Chinese
-
Abstract:
The study aimed to investigate the effects of small ubiquitin-related modifier (SUMO) specific protease 1 (SENP1) on human PXR-mediated MDR1 transcriptional activity and mRNA expression. Empty vector and expression plasmids, including PXR, SENP1 and SENP1 mutant (SENP1m) were transiently transfected into HepG2 and LS174T cells using Lipo2000. Transcriptional activity was detected by dual luciferase reporter gene assay, and mRNA level was measured using real-time polymerase chain reaction. The results showed that SENP1 could remarkably reduce the rifampicin (RIF)-induced MDR1 reporter activity and mRNA level in hPXR over expressed HepG2 and LS174T cells (P < 0.05), whereas adding SENP1m restored the RIF-induced increases (P < 0.05). These results indicated that SENP1 could repress the RIF-induced hPXR-mediated MDR1 transcriptional activity and mRNA expression.
