Application of TRUST, TP-ELISA and TPPA in the serologic diagnosis of syphilis.
- Author:
Ting-Ting WANG
1
;
Qin LI
;
Jing-Feng YU
;
Chen-Yue XIAO
;
Li-Ping ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; methods; Humans; Infant; Infant, Newborn; Middle Aged; Syphilis; blood; diagnosis; Syphilis Serodiagnosis; methods; Treponema pallidum; Young Adult
- From: National Journal of Andrology 2012;18(11):1020-1022
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the application value of toluidine red unheated serum test (TRUST), treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA) and treponema pallidum particle agglutination assay (TPPA) in the serologic diagnosis of syphilis.
METHODSWe collected serum samples from 12 622 inpatients, 2 253 outpatients and 1 500 subjects in the medical examination center, and screened them by TRUST and TP-ELISA, followed by quantitative tests using TRUST and TPPA.
RESULTSAmong the 16 375 cases, clinically diagnosed syphilis was confirmed in 384, with a positive rate of 100% either by TRUST (1:2 -1 :16) or TPPA (1:80 - 1:2 560), and 92.2% by TP-ELISA.
CONCLUSIONTP-ELISA and TRUST should be simultaneously used for syphilis screening, but not TP-ELISA alone, and the results should be confirmed by TPPA. The combination of the three methods plays a valuable role in reducing the misdiagnosis and missed diagnosis, and contributes to the early and accurate diagnosis of syphilis.
