Sperm DNA damage and sperm-nucleoprotein transition correlate to acrosin activity and seminal parameters.

Jiu-jia Zheng; Xu Yang; Li-ya Zhang; Qian-jin Fei; Cheng-shuang Pan; Wu-hua NI; Jian-yuan Jin; Xue-feng Huang

Return

Sperm DNA damage and sperm-nucleoprotein transition correlate to acrosin activity and seminal parameters.

Author: Jiu-Jia ZHENG ¹ ; Xu YANG ; Li-Ya ZHANG ; Qian-Jin FEI ; Cheng-Shuang PAN ; Wu-Hua NI ; Jian-Yuan JIN ; Xue-Feng HUANG
Author Information

1. Reproductive Medicine Center, The First Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, China. zhengjiujia@126.com
Publication Type:Journal Article
MeSH: Acrosin; genetics; Adult; Chromatin; DNA Damage; DNA Fragmentation; Humans; Infertility, Male; genetics; Male; Nucleoproteins; genetics; metabolism; Sperm Count; Sperm Motility; Spermatozoa
From: National Journal of Andrology 2012;18(10):925-929
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters.
METHODSWe collected 535 semen samples, assessed sperm DNA damage by sperm chromatin dispersion test, and analyzed the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters according to the WHO criteria.
RESULTSStatistically significant differences were observed in sperm DNA damage among sperm-nucleoprotein transition, acrosin activity, sperm concentration and the percentage of grade a + b sperm (P < 0.01). Sperm DNA damage was positively correlated with age, sperm-nucleoprotein transition, sperm concentration and the percentage of grade d sperm (P < 0.01 or P < 0.05), but negatively correlated with acrosin activity (P < 0.001). Stepwise linear regression analysis demonstrated that age, sperm concentration, the percentage of grade d sperm, sperm-nucleoprotein transition and acrosin activity were independent variables related to the DNA fragmentation index (DFI). The abnormality rates of sperm-nucleoprotein transition, acrosin activity, sperm concentration and graded a + b sperm were significantly higher in the sperm DNA damage group (DFI > or = 30%) than in the normal control (DFI < 30%) (P < 0.01).
CONCLUSIONSperm DNA damage is closely related with sperm-nucleoprotein transition, acrosin activity and seminal parameters, which may become another important independent parameter for the evaluation of sperm quality.