Effects of serum anti-sperm antibodies positive on the Fas/Fas-L apoptosis pathway in the testis tissue and testicular germ cells of pubertal male rats.
- Author:
Er-Lei ZHI
1
;
Ji-Xiu XU
;
Jing-Qi WANG
;
Ning XU
;
Peng JI
;
Zhuo WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Autoantibodies; immunology; Caspase 3; metabolism; Fas Ligand Protein; metabolism; Germ Cells; cytology; immunology; metabolism; Infertility, Male; Male; Rats; Rats, Wistar; Signal Transduction; Testis; cytology; metabolism; fas Receptor; metabolism
- From: National Journal of Andrology 2013;19(2):111-115
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a rat model of anti-sperm antibody (AsAb)-mediated immune infertility, and investigate the effects of serum AsAb positive on the Fas/Fas-L apoptosis pathway in testis tissue and testicular germ cells of pubertal male rats.
METHODSThirty 5-week-old Wistar male rats were included in this study, 10 killed for preparation of sperm suspension, 10 as normal controls, and the other 10 made models of AsAb-positive immune infertility (experimental group). Four weeks after modeling, the testes of the rats were harvested for observation of the changes in the testis tissue under the light microscope and detection of the expressions of Fas, Fas-L and Caspase-3 proteins by immunohistochemistry.
RESULTSCompared with the control group, the experimental group showed obvious apoptotic changes in the testis tissue and remarkably increased expressions (OD value) of Fas (161.87 +/- 5.37 vs 176.97 +/- 4.58), Fas-L (150.27 +/- 8.65 vs 187.52 +/- 7.76) and Caspase-3 (120.37 +/- 6.76 vs 157.65 +/- 7.38) (P < 0.01).
CONCLUSIONSerum AsAb affected the infertility of pubertal male rats, and its mechanisms might be associated with up-regulated expression of Fas, Fas-L and Caspase-3 proteins in the Fas/Fas-L apoptotic pathway.
