Analysis of the protein expression and gene amplification of HER2 in gastric cancer.
- Author:
Jun-lin LIU
1
;
Zhen-kai LI
;
Fang-jie XIN
;
Han ZHAO
;
Wei ZHAO
;
Yuan GAO
;
Ji-lin HU
;
Xian-xiang ZHANG
;
Mao-shen ZHANG
;
Bing-yuan ZHANG
;
Yun LU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Gene Amplification; Humans; Male; Middle Aged; Receptor, ErbB-2; genetics; metabolism; Stomach Neoplasms; genetics; metabolism; pathology
- From: Chinese Journal of Gastrointestinal Surgery 2013;16(9):898-901
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the clinical significance of protein expression and gene amplification of HER2 in gastric cancer.
METHODSImmunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) method were used to detect protein expression and gene amplification of HER2 in 80 specimens of gastric cancer patients.
RESULTSProtein expression of HER2 was negative in 51 cases, (+) in 12 cases, (++) in 12 cases, (+++) in 5 cases, and the positive expression rate was 21.3% (17/80). Seven (8.8%) cases had gene amplification of HER2, including gene critical amplification in 3 (3.8%) cases. The result of IHC was positively correlated with CISH (P<0.05), and the coincidence rate was 85.0% (68/80). HER2 positive expression rate was higher in the gastroesophageal junction carcinoma, poorly differentiated and stage III-IIII gastric cancer (all P<0.05).
CONCLUSIONThe gastric cancer tissue has high positive rate of protein expression and gene amplification of HER2, which is closely correlated to the development of gastric cancer.
