Study on miR-490-5p and miR-363 as novel biomarkers for the diagnosis of colorectal cancer.
- Author:
Xuehu XU
1
;
Xiaobing WU
;
Shangbiao WU
;
Qingping JIANG
;
Haibo LIU
;
Rong CHEN
;
Yan SUN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; analysis; Case-Control Studies; Colorectal Neoplasms; diagnosis; Early Detection of Cancer; Female; Humans; Male; MicroRNAs; analysis; Middle Aged; Sensitivity and Specificity; Young Adult
- From: Chinese Journal of Gastrointestinal Surgery 2014;17(1):45-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify dysregulated microRNA(miRNA) that can act as novel biomarker for colorectal cancer screening.
METHODSReal time-polymerase chain reaction was used to detect the expression profile of miRNA in tumor and paired normal tissues, and the significant dysregulated miRNA was examined by non-paired t-test. Receiver operating characteristic(ROC) curve was performed to evaluate the specificity and sensitivity of miR-363 and miR-490-5p as biomarkers to discriminate colorectal cancer patients from normal person.
RESULTSSeven-three dysregulated miRNAs were found in male samples, of which 6 miRNAs were up-regulated and other 67 miRNAs were down-regulated, while 42 dysregulated miRNAs were found in female samples, of which 5 were up-regulated and 37 were down-regulated in tumor tissues compared with normal tissues. Among above dysregulated miRNAs, 33 miRNAs had significant differences, besides, dysregulated expression level of 10 of 33 miRNAs was over 5 folds in male and female as well as mixed samples.
CONCLUSIONSThe expression pattern in female is different from that in male. miR-363 and miR-490-5p possess the potential in screening colorectal cancer patients from healthy people.