Cloning and expression offrom.
- Author:
Dongping TU
1
;
Xiaojun MA
2
;
Huan ZHAO
1
;
Changming MO
3
;
Qi TANG
4
;
Liuping WANG
5
;
Jie HUANG
5
;
Limei PAN
3
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Peking 100193, China.
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Peking 100193, China
3. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
4. Horticulture & Landscape College, Hunan Agriculture University, Changsha 410128, China.
5. Guangxi University of Chinese Medicine, Nanning 530001, China.
- Publication Type:Journal Article
- Keywords:
Auxin;
CYP87A3;
Expression pattern;
Race clone;
SgCYP450-4;
Siraitia grosvenorii
- From:
Acta Pharmaceutica Sinica B
2016;6(6):614-622
- CountryChina
- Language:English
-
Abstract:
CYP450 plays an essential role in the development and growth of the fruits of. However, little is known about thegene in. Here, based on transcriptome data, a full-length cDNA sequence ofwas cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) strategies.is 1677 bp in length (GenBank accession No. AEM42985.1) and contains a complete open reading frame (ORF) of 1422 bp. The deduced protein was composed of 473 amino acids, the molecular weight is 54.01 kDa, the theoretical isoelectric point (PI) is 8.8, and the protein was predicted to possess cytochrome P450 domains.gene was highly expressed in root, diploid fruit and fruit treated with hormone and pollination. At 10 days after treatment with pollination and hormones, the expression of Sghad the highest level and then decreased over time, which was consistent with the development of fruits of. Hormonal treatment could significantly induce the expression of. These results provide a reference for regulation of fruit development and the use of parthenocarpy to generate seedless fruit, and provide a scientific basis for the production of growth regulator application agents.
