Analysis of Cordyceps by multi-column liquid chromatography.
10.1016/j.apsb.2016.10.002
- Author:
Zhengming QIAN
1
,
2
;
Shaoping LI
3
Author Information
1. Institute of Chinese Medical Sciences, University of Macau, Macao, China
2. Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co., Ltd., Dongguan 523850, China.
3. Institute of Chinese Medical Sciences, University of Macau, Macao, China.
- Publication Type:Journal Article
- Keywords:
Cordyceps;
Macromolecules;
Micromolecules;
Multi-column;
Size exclusion column
- From:
Acta Pharmaceutica Sinica B
2017;7(2):202-207
- CountryChina
- Language:English
-
Abstract:
Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion pre-column, a size exclusion column (SEC) and a reversed phase column (RP) which were controlled by column-switching valves. The sample was separated by the size exclusion pre-column into two fractions (macromolecules and micromolecules). These fractions were further separated on SEC and RP columns, respectively. A diode array detector (DAD) and a mass spectrometer (MS) were used to detect the components. This MC-LC method was utilized for analysis of Cordyceps samples. Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps, and 11 of the micromolecular peaks were identified as adenosine-5'-monophosphate (AMP), phenylalanine, uridine, hypoxanthine, inosine, guanine, guanosine, deoxyadenosine-5'-monophosphate (dAMP), adenosine, adenine and cordycepin (or its isomer). This method is useful for quality control of Cordyceps.
