Quantitative Analysis of DNA Single-strand Breaks in EL 4 cells and Mouse Spleen Lymphocytes after Irradiation.
- Author:
Seoun Yul YOO
1
;
Chul Koo CHO
;
Kyung Hwan KOH
;
Woo Yoon PARK
;
Young Hwan PARK
;
Sung Ho KIM
;
Tae Hwan KIM
;
In Yong CHUNG
Author Information
1. Department of Therapeutic Radiology, KCCH, KAERI, Korea.
- Publication Type:Original Article
- Keywords:
Alkaline elution;
EL 4;
Lymphocytes;
Single strand breaks;
Filter
- MeSH:
Animals;
DNA*;
Hydrogen-Ion Concentration;
Leukemia;
Lymphocytes*;
Mice*;
Radiation, Ionizing;
Spleen*;
Thymidine
- From:Journal of the Korean Society for Therapeutic Radiology
1990;8(2):137-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
The filter elution technique was used to assay Co-60 g ray-induced DNA single-strand breaks (SSB) in EL 4 mouse leukemia cell and spleen lymphocyte. The lymphocytes were stimulated with lipopolysaccharide (LPS, 20 mug/ml) to label [3H] thymidine. EL 4 cells and lymphocytes in suspension were exposed at 0degree C to gy, 1 gy, 5 gy, 10 gy of Co-60 radiation and elution procedure was performed at PH 12.1. The number of DNA single-strand breaks increased with increasing doses of g rays. The strand scission factor (SSF) was estimated in each experiment (eluted volume 21 ml. The slope for EL 4 cells was 0.01301+/-0.00096 gy-1(n=5) and the slope for lymphocytes was 0.01097+/-0.00091 gy-1(n=5). The slopes were significantly different (p<0.005). Thus EL 4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes.
