Fabrication of a blood vessel scaffold with a combined polymer for tissue engineering.
- Author:
Yong PAN
1
;
Wei HUANG
;
Yu-feng AI
;
Meng XIONG
;
Lin-xi ZHANG
;
Pai PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Vessels; growth & development; Collagen; Muscle, Smooth, Vascular; growth & development; Polyglycolic Acid; Polymers; Rabbits; Time Factors; Tissue Engineering; instrumentation; methods; Tissue Scaffolds
- From: Chinese Journal of Plastic Surgery 2003;19(1):44-46
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possibility to fabricate a blood vessel scaffold with a combined polymer for tissue engineering.
METHODSA blood vessel scaffold was designed with a combined polymer composed of rabbit vascular smooth muscle cells(VSMCs), collagen and a non-spinning fabric mesh of polyglycolic acid (PGA). VSMCs were implanted into collagen gel and their growth was observed. The mixed solution of VSMCs and collagen was dropped into the tubular scaffold, followed by 7-day culturing.
RESULTSVSMCs formed many prominences after culturing in gelatinous collagen for 3-4 hours. With cells extending, some cells became shuttle- or spindle-shaped. After VSMCs-collagen complex was implanted into the PGA mesh, most of VSMCs remained in the pore of PGA mesh with the formation of gelation. VSMCs could adhere to and grow on the PGA fiber.
CONCLUSIONThe non-spinning PGA porous biodegradable material coated with collagen is a good carrier for VSMCs to adhere and grow.
