Experimental study on effects of endothelin in the proliferation and collagen synthesis of human scar-derived fibroblasts.
- Author:
De-wu LIU
1
;
Guo-hui LI
Author Information
- Publication Type:Journal Article
- MeSH: Benzylisoquinolines; pharmacology; Cell Proliferation; drug effects; Cells, Cultured; Cicatrix; pathology; Collagen; biosynthesis; DNA; biosynthesis; Endothelins; antagonists & inhibitors; pharmacology; Fibroblasts; cytology; radiation effects; Humans; Nitric Oxide; metabolism; pharmacology; Proline; metabolism; S-Nitroso-N-Acetylpenicillamine; pharmacology
- From: Chinese Journal of Plastic Surgery 2003;19(1):51-53
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of endothelin (ET) in the proliferation and collagen synthesis of human scar-derived fibroblasts and the modulation of its antagonists such as nitric oxide (NO), tetrandrine (Tet).
METHODSWith the cultured fibroblasts from the scarring tissue, the cell proliferation was determined by [3H]-TdR incorporation, while the collagen synthesis was evaluated by [3H]-proline incorporation.
RESULTSThe ET-1 was significantly increasing the proliferation and collagen synthesis of human scar-derived fibroblasts. The values of [3H]-TdR absorption in the 2.5 ng/ml, 25 ng/ml and 100 ng/ml of ET-1 groups were 1.8 times, 4 times and 4.9 times more than in the control group, respectively (P < 0.01), while the values of the [3H]-proline incorporation were 1.1 times, 3.1 times and 3.8 times respectively (P < 0.01). The fibroblasts, treated with 50 micrograms/ml of S-nitroso-N-acetyl penicillamine(SNAP), were no detectable effect on the basal level of DNA synthesis, but produced decreasing effect on the [3H]-TdR absorption (the rate of inhibition was 22.89%, P < 0.05). It was found that the SNAP inhibited the [3H]-proline incorporation in cultured fibroblasts, but the rate of [3H]-proline incorporation induced by ET-1 was unaltered. The Tet with 3 micrograms/ml, in which does not inhibit the basal level of DNA synthesis, was significantly decreasing the collagen synthesis and decreasing the ET-mediated DNA synthesis (the rate of inhibition was 33.21% (P < 0.01).
CONCLUSIONThese results indicate that the ET can obviously increase the proliferation and collagen synthesis of human scar-derived fibroblasts, but it can be partially antagonized by NO and Tet.