The effect of liposome-carried metallothionein on secondary venous ischemia-reperfusion injury in a rat flap.
- Author:
Jie GUO
1
;
Dong-yan WANG
;
De-hong GUAN
;
Chao-shu TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Endothelins; blood; Graft Survival; Ischemia; metabolism; Lactate Dehydrogenases; blood; Liposomes; Malondialdehyde; metabolism; Metallothionein; administration & dosage; Peroxidase; metabolism; Rats; Rats, Wistar; Reperfusion Injury; blood; prevention & control; Surgical Flaps; blood supply; Time Factors
- From: Chinese Journal of Plastic Surgery 2003;19(4):245-247
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of liposome-carried metallothionein (lipo-MT) on secondary ischemia-reperfusion injury in the rat island flap.
METHODSAn abdominal island flap was created in the Wistar rat. The animals were divided into four groups: the sham group, the secondary ischemia-reperfusion group, the group treated with blank liposome and the group treated with lipo-MT. The malondialdehyde (MDA) content, the myeloperoxidase (MPO) activity was assayed immediately, at 30 minutes and 7 days after the secondary venous ischemia-reperfusion. The level of endothelin (ET) and lactic dehydrogenase (LDH) of the rat plasma was measured at 30 minutes after secondary venous ischemia-reperfusion. The content of MT of the flap was assayed by Cd-hemoglobin saturation method at 7 days after the operation.
RESULTSThe treatment of lipo-MT significantly decreased the content of MDA, MPO of the flap, decreased the activity of ET, LDH of the rat palsma, increased the content of MT of the flap and improved the flap viability.
CONCLUSIONLipo-MT can improve flap survival by reducing ischemia-reperfusion injury.
