Investigation of p53 gene mutations in keloids using PCR-SSCP.
- Author:
Yong-bo LIU
1
;
Jian-hua GAO
;
Hong-jie DUAN
;
Xiao-jun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Base Sequence; Exons; genetics; Fibroblasts; Genes, p53; Humans; Keloid; genetics; Mutation; Polymerase Chain Reaction; methods; Polymorphism, Single-Stranded Conformational; Sequence Analysis, DNA; Tumor Suppressor Protein p53; genetics
- From: Chinese Journal of Plastic Surgery 2003;19(4):258-260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect gene mutations of p53 gene (exon 4-6) in fibroblasts.
METHODSSamples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing.
RESULTSGene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids.
CONCLUSIONGene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis.
