The regulating effect of antisense-S-Oligo on TYR gene expression and melanin production of melanocytes.
- Author:
Yu-guang ZHANG
1
;
Qiong-hua HU
;
Xun-zhang WANG
;
Zuo-liang QI
;
Xiao-xi LIN
;
Jian-lin FANG
;
Chuan-chang DAI
Author Information
- Publication Type:Journal Article
- MeSH: 3' Flanking Region; genetics; 5' Flanking Region; genetics; Endothelins; pharmacology; Gene Expression; Melanins; biosynthesis; Melanocytes; drug effects; metabolism; radiation effects; Oligodeoxyribonucleotides, Antisense; genetics; pharmacology; Tyrosine; genetics; metabolism; Ultraviolet Rays
- From: Chinese Journal of Plastic Surgery 2003;19(4):285-287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEDespite the causes for melanin increase, the increased gene expression of TYR is a common pathological process. Based on this viewpoint, antisense-S-Oligo of TYR was designed and synthesized to regulate synthesis of melanin in order to explore the treatment for skin pigmentation.
METHODSThe cultured melanocytes were divided into 3 groups. The group 1 was treated with endothelin, group 2 treated with ultraviolet ray and group 3 was used as the control. In each group, the 5' antisense-S-Oligo, the 3' antisense-S-Oligo, the mixed antisense-S-Oligo of TYR or Dotap only was added. The melanin content and TYR gene expressions were examined.
RESULTSThe 5' antisense-S-Oligo, the 3' antisense-S-Oligo and the mixed antisense-S-Oligo significantly inhibited the increase of melanin content and TYR gene expression, which were caused by endothelin or ultraviolet ray treatment. Of the three treatments, the 3' antisense-S-Oligo showed the strongest effect.
CONCLUSIONAntisense-S-Oligo has significant regulating effects on TYR gene expression and melanin content. The 3' antisense-S-Oligo is more effective than the 5' antisense-S-Oligo.
