Effect of tanshinone microemulsion on reversing MDR in human tumor cells.
- Author:
Qing FAN
1
;
Guang-jun FAN
;
Pei-man YANG
;
Jin-yao ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antineoplastic; pharmacology; Antineoplastic Agents, Phytogenic; administration & dosage; pharmacology; Cell Proliferation; drug effects; Diterpenes, Abietane; Doxorubicin; pharmacology; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; Drugs, Chinese Herbal; administration & dosage; pharmacology; Emulsions; Humans; K562 Cells; drug effects; Phenanthrenes; administration & dosage; pharmacology
- From: China Journal of Chinese Materia Medica 2004;29(11):1079-1081
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of tanshinone microemulsion (Tan-M) on the cytotoxicity to human leukemia-cell-line (K562/ADM) and the reversion of MDR in vitro.
METHODMicroemulsion being supposed as the control group, MT method is adopted to test cytotoxicity and the reverse of MDR.
RESULTObvious cytotoxicity to K562/ADM was observed for tan-M. Cell non-toxic dosage (growth quotiety > 95%) of Tan-M is 0.2 microg x mL(-1). Low toxic dosage (growth quotiety 85-90%) was 0.7 microg x mL(-1). Cell non-toxic dosage of was 0.7 microg x mL(-1) and low toxic dosage was 1.2 microg x mL(-1). Cell non-toxic dosage of Tan-M (0.2 microg x mL(-1)) significantly lowered the IC50 of K562/ADM by ADM (P < 0.01), and reversed MDR was 3.88 times. Low toxic dosage of Tan-M reversed MDR was 3.97 times. E-M (0.2 microg x mL(-1)) reversed MDR was 2.62 times.
CONCLUSIONThe result indicates that tanshinone microemulsion possesses cell-toxic effects on human leukemia cell-line and may reverse MDR of tumor cells.
