Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion.
- Author:
Deng-ming WEI
1
;
Guang-zhao HUANG
;
Yi-gu ZHANG
;
Guang-xun RAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Brain Ischemia; pathology; Diterpenes; isolation & purification; pharmacology; Epoxy Compounds; Female; Infarction, Middle Cerebral Artery; pathology; Male; Neuroprotective Agents; pharmacology; Neutrophil Infiltration; drug effects; Phenanthrenes; isolation & purification; pharmacology; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Reperfusion Injury; pathology; Tripterygium; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(11):1089-1116
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study effect of triptolide (TL) on neuronal apoptosis in cerebral tissue of rat after ischemia-reperfusion.
METHODTriptolide at dose 0.2 or 0.4 mg x kg(-1) was intraperitoneally injected once a day for 4 d. The focal cerebral ischemia-reperfusion model was established with thread embolism in middle artery before triptolide injection on the fourth day. Neurological deficit score of rats was evaluated; and immunohistochemical techniques were used to count positive cells of express of MPO and TUNEL in cerebraltissue.
RESULTCompared with the control group, the deficit of neural function was significantly improved, and the number of infiltrate of neutrophil and neuronal apoptosis in cerebral tissue was remarkably reduced in two TL-treated groups.
CONCLUSIONThe results suggested that TL can inhibit infiltration of neutrophil and decrease the degree of neuronal apoptosis in cerebral tissue.
